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Math. 11 item. is renamed in 1970 to Mathematics for Managers. 4 item.Stress Induced Modulation of the Ammonium Affinity of a Bacterial Chaperone: The Case of StmK. The cation permeases NhaA and CzcD are both thermostabilized in the presence of ammonium ions (NH4+). This stabilizing effect is only observed when the proteins are in their native conformation (at pH 5-8) and only if the cation is coordinated to a lysine of the protein. Recently, we have identified and characterized the protein StmK (f.i. G = glutamine) as a yet unidentified factor in Salmonella that counteracts the thermostabilizing effects of NH4+ on NhaA and CzcD. In this study, we will focus on the molecular basis of this process. Specifically, we will determine if the observed effect on the ammonium affinity of NhaA is caused by direct interaction with the ammonium cation or with an allosteric site of the protein. Also, we will assess whether or not StmK is able to interact with NhaA and CzcD at different salt concentrations. Based on our initial structural model for StmK, we will also search for a potential allosteric site in the protein and test its involvement in the counteracting effect of StmK on ammonium affinity. Finally, we will confirm StmK's contribution to the ammonium resistance phenotype of Salmonella and define its cellular role by creating an in-frame deletion mutant.Pupils at an inner London school, where 70 per cent are on free school meals, were forced to use their meagre pocket money to pay a school bus driver who then pocketed £6,000 in cash, a court heard today. In a number of alleged offences, the 24-year-old driver - who has not been named - pocketed money earned by 10 children between the ages of eight and 16 at Plumstead Academy in Waltham Forest, north-east London. The former unemployed bus driver was arrested after the school, in Plumstead, was alerted to the fraud. She is accused of two offences of theft from a person and two offences of theft of a motor vehicle. A jury at the Old Bailey heard that the driver, who was working for the council, was stealing the cash at the end of




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